Radioimmunoassay for progesterone, testosterone and estradiol-17.BETA. using 125I-iodohistamine radioligands.
نویسندگان
چکیده
منابع مشابه
Biodegradation of 17beta-estradiol, estrone and testosterone in stream sediments.
Biodegradation of 17beta-estradiol (E2), estrone (E1), and testosterone (T) was investigated in three wastewater treatment plant (WWTP) affected streams in the United States. Relative differences in the mineralization of [4-(14)C] substrates were assessed in oxic microcosms containing saturated sediment or water-only from locations upstream and downstream of the WWTP outfall in each system. Ups...
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Control of mitochondrial biogenesis in brown adipose tissue (BAT), as part of the thermogenesis program, is a complex process that requires the integration of multiple transcription factors to orchestrate mitochondrial and nuclear gene expression. Despite the knowledge of the role of sex hormones on BAT physiology, little is known about the effect of these hormones on the mitochondrial biogenic...
متن کامل125I-labeled radioimmunoassay kits for progesterone evaluated for use in an vitro fertilization program.
We have evaluated two commercially available 125I radioimmunoassay kits (Diagnostic Products Corp., DPC; and Radioassay Systems Laboratories, RSL) for measurement of serum or plasma progesterone, to determine their suitability for use in in vitro fertilization programs. Both kits were suitably rapid for program requirements. Results by both were linear with concentration up to 60 nmol/L, and bo...
متن کاملSpecific binding sites for progesterone and 17beta-estradiol in cells of Triticum aestivum L.
The presence and location of specific binding sites for progesterone and 17beta-estradiol in cells of wheat were estimated using radioligand binding assay. Membrane and cytosolic fractions of non-vernalized and vernalized plants were tested using tritium-labelled ligands. Specific binding of [(3)H]progesterone and [(3)H]17beta-estradiol occurs in wheat cells. The binding sites are located in me...
متن کاملA strategy for radioimmunoassay of plasma progesterone with use of a homologous-site 125I-labeled radioligand.
In this sensitive, specific 125I radioimmunoassay for plasma progesterone, an identical glucuronide bridge is used in the immunogen and radioligand. This apparently provides a system in which the bridge is poorly recognized and thereby avoids the excessively high affinity of the tracer for the antiserum and the consequent poor sensitivity that commonly is found in other homologous-bridge situat...
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ژورنال
عنوان ژورنال: The Japanese journal of animal reproduction
سال: 1985
ISSN: 0385-9932
DOI: 10.1262/jrd1977.31.186